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KMID : 0861020150300010059
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Korea Journal of Herbology
2015 Volume.30 No. 1 p.59 ~ p.65
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Effects of Polygalae Radix on Apotosis in PC-12 Cell
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Lee Sang-Chul
Kim Youn-Sub
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Abstract
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Objectives : The purpose of this study was to observe the effects of Polygalae Radix(PR) on 4-HNE-induced apoptosis in PC-12 cell.
Methods : A MTT assay was conducted to observe the cytotoxicity of Polygalae Radix on the cell viability and the cytoprotective effect of Polygalae Radix against 4-HNE that causes oxidative stress-induced cytotoxicity, and then a western blot was conducted to observe the expression of TNF-α, caspase-3, Bax and Bcl-2 protein that are important factors involved with apoptosis signaling pathway.
Results : The Polygalae Radix water extract 25 ㎍, 50 ㎍, 100 ㎍and 200 ㎍/mL had no cytotoxicity on the PC-12 cell. The Polygalae Radix water extract 25 ㎍, 50 ㎍and 100 ㎍/mL had the cytoprotective effect against 4-HNE that causes cytotoxicity on the PC-12 cell. The Polygalae Radix water extract 50 ㎍/mL significantly suppressed the increase in TNF-α protein expression in PC-12 cell. The Polygalae Radix water extract 25 ㎍and 50 ㎍/mL significantly suppressed the increase in caspase-3 protein expression in PC-12 cell. The Polygalae Radix water extract 25 ㎍, 50 ㎍and 100 ㎍/mL suppressed the increase in Bax protein expression in PC-12 cell but had no significance. The Polygalae Radix water extract 25 ㎍and 100 ㎍/mL significantly prevented the decrease in Bcl-2 protein expression in PC-12 cell,
Conclusions : These results suggest that the Polygalae Radix water extract is effective in inhibiting apoptosis.
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KEYWORD
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Polygalae Radix, 4-HNE, TNF-α, caspase-3, Bax, Bcl-2
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